4RX6
Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.200, 103.200, 77.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.687 - 2.599 |
| R-factor | 0.2289 |
| Rwork | 0.224 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r25 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.541 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.700 |
| High resolution limit [Å] | 2.599 |
| Number of reflections | 14872 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 4000, PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
