4RX1

Crystal Structure of antibiotic-resistance methyltransferase Kmr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	193
Detector technology	CCD
Collection date	2011-01-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	89.506, 89.506, 127.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.350 - 2.472
R-factor	0.2162
Rwork	0.214
R-free	0.26310
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.470
Number of reflections	21690
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	277	100 mM MES buffer pH 6.1, 6% PEG MME 5000, 8% 1-propanol, 0.5 mM SAM, 100 mM sodium iodide, VAPOR DIFFUSION, SITTING DROP, temperature 277K