4RWZ
Crystal structure of the antibiotic-resistance methyltransferase Kmr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-04-22 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.643, 89.643, 126.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.102 - 1.801 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 54737 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 mM HEPES buffer pH 7.5, 22% PEG MME 2000, 200 mM potassium thiocyanate, cryoprotected with 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
