4RWW
Crystal Structure of L. monocytogenes PstA in complex with cyclic-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 51.108, 51.788, 52.996 |
Unit cell angles | 110.80, 105.59, 109.84 |
Refinement procedure
Resolution | 29.580 - 1.600 |
R-factor | 0.15909 |
Rwork | 0.157 |
R-free | 0.19446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m05 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.693 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.090 | 0.416 |
Number of reflections | 54378 | |
<I/σ(I)> | 14.7 | 3.2 |
Completeness [%] | 96.6 | 94.6 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |