4RWE
The crystal structure of a sugar-binding transport protein from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.940, 59.940, 169.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.660 - 1.650 |
| R-factor | 0.1644 |
| Rwork | 0.161 |
| R-free | 0.22570 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.929 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.660 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.077 | 0.764 |
| Number of reflections | 37067 | |
| <I/σ(I)> | 24.1 | 1.2 |
| Completeness [%] | 96.8 | 89.2 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.8M Ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
