4RW0
Crystal structure of a member of the lipolytic protein G-D-S-L family from Veillonella parvula DSM 2008
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 63 |
Unit cell lengths | 121.650, 121.650, 84.173 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.000 - 2.000 |
R-factor | 0.15881 |
Rwork | 0.157 |
R-free | 0.18628 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.759 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | |
Number of reflections | 47389 | |
<I/σ(I)> | 19 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.0 M K/Na tartrate, 0.2M Li2SO4, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |