4RVZ
Crystal structure of tRNA fluorescent labeling enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-07-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.623, 69.623, 211.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.066 - 2.900 |
R-factor | 0.214 |
Rwork | 0.210 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | selenomethionine derived Tias coordinate |
RMSD bond length | 0.008 |
RMSD bond angle | 1.263 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.426 | |
Number of reflections | 11960 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 50 mM HEPES, pH 7.0-7.2, 0.5 M NH4Ac, 0.02 M MgCl2, 1.5-2.5% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |