4RVS
The native structure of mycobacterial quinone oxidoreductase Rv154c.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | H 3 |
| Unit cell lengths | 83.518, 83.518, 273.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.591 - 1.846 |
| R-factor | 0.1701 |
| Rwork | 0.170 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qor |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rmerge | 0.070 | 0.169 |
| Number of reflections | 60920 | |
| <I/σ(I)> | 12.6 | 12.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28 % v/v PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
