4RVQ

PWI-like domain of Chaetomium thermophilum Brr2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-25
Detector	MAR CCD 165 mm
Wavelength(s)	0.91841
Spacegroup name	C 1 2 1
Unit cell lengths	123.846, 45.752, 27.420
Unit cell angles	90.00, 102.56, 90.00

Refinement procedure

Resolution	26.764 - 1.135
R-factor	0.1254
Rwork	0.124
R-free	0.14580
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.477
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.790	1.200
High resolution limit [Å]	1.135	1.140
Number of reflections	54190
<I/σ(I)>	13	2.9
Completeness [%]	100.0	92.8
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP	8	277	1.9 M ammonium sulfate, 0.1 M Tris-HCl, 0.01 M Cobalt chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K