4RVB

Crystal Structure Analysis of the Human Leukotriene A4 Hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX MX225HE
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	77.939, 87.153, 99.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.270 - 1.930
R-factor	0.14585
Rwork	0.144
R-free	0.17835
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ftx
RMSD bond length	0.019
RMSD bond angle	1.820
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.270	1.983
High resolution limit [Å]	1.930	1.933
Number of reflections	48698
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	13% PEG 8000, 100MM IMIDAZOLE PH 6.2, 100MM NAACETATE, 5MM YBCL3, VAPOR DIFFUSION, HANGING DROP, temperature 293K