4RV1
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium (NESG) Target OR497.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97907 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.265, 99.265, 432.183 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.020 - 2.573 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.23700 |
Structure solution method | MIR |
Starting model (for MR) | 4hxt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.148 | 0.818 |
Number of reflections | 148422 | |
<I/σ(I)> | 15.2 | 2.01 |
Completeness [%] | 98.2 | 100 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under paraffin oil | 7.5 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 40% PEG 400, 0.1M calcium acetate, 0.1M sodium acetate, 3% ethylene glycol , microbatch under paraffin oil, temperature 291K |