4RUW
The crystal structure of endonuclease/exonuclease/phosphatase from Beutenbergia cavernae DSM 12333
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.995, 81.121, 46.854 |
| Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
| Resolution | 26.393 - 1.281 |
| R-factor | 0.1383 |
| Rwork | 0.138 |
| R-free | 0.14810 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1683)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.082 | |
| Number of reflections | 114702 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 4.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M Sodium Acetate:HCl pH 4.6 8% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
