4RUQ
Carp Fishelectin, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.310, 70.990, 163.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.18501 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.089 | |
| Number of reflections | 113338 | |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 10% 2-propanol, 20% PEG 4000, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
