4RUC

Crystal structure of Y-family DNA polymerase Dpo4 extending from a MeFapy-dG:dC pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0781
Spacegroup name	P 21 21 2
Unit cell lengths	94.383, 103.972, 52.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.880 - 2.900
R-factor	0.17482
Rwork	0.172
R-free	0.23561
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.451
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER ((MR))
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	3.540	2.950
High resolution limit [Å]	2.900	3.440	2.900
Rmerge	0.143	0.269	0.617
Number of reflections	11809
<I/σ(I)>	18.715	10.059	1.81
Completeness [%]	99.8	100	100
Redundancy	7.1	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	50 MM TRIS HCL, PH 7.4, 15% PEG3350, 100MM CALCIUM DIACETATE, 2.5% GLYCEROL , VAPOR DIFFUSION, HANGING DROP, temperature 298K