4RU5
Crystal Structure of the Pseudomonas phage phi297 tailspike gp61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.617, 124.705, 83.563 |
| Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
| Resolution | 46.370 - 1.520 |
| R-factor | 0.08081 |
| Rwork | 0.079 |
| R-free | 0.11953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.784 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.470 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.038 | 0.300 |
| <I/σ(I)> | 16.8 | 3.8 |
| Completeness [%] | 98.1 | 94.8 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 17% Glycerol, 11% PEG 4000, 0.1M Hepes pH 7.0 15 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
