4RU5
Crystal Structure of the Pseudomonas phage phi297 tailspike gp61
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 210.617, 124.705, 83.563 |
Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
Resolution | 46.370 - 1.520 |
R-factor | 0.08081 |
Rwork | 0.079 |
R-free | 0.11953 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.784 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.470 | 1.600 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.038 | 0.300 |
<I/σ(I)> | 16.8 | 3.8 |
Completeness [%] | 98.1 | 94.8 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 17% Glycerol, 11% PEG 4000, 0.1M Hepes pH 7.0 15 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 291K |