4RU4
Crystal structure of the tailspike protein gp49 from Pseudomonas phage LKA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.400, 148.234, 117.582 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 19.992 - 1.903 |
| R-factor | 0.1315 |
| Rwork | 0.129 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.113 | 0.457 |
| Number of reflections | 304934 | |
| <I/σ(I)> | 10.6 | 3.4 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 291 | 8% PEG 6000, 0.8M NaCl, 0.1M Na acetate pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
