4RU0
The crystal structure of abc transporter permease from pseudomonas fluorescens group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 32 |
| Unit cell lengths | 99.181, 99.181, 104.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.748 - 2.442 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21460 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1683)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 68079 | |
| <I/σ(I)> | 15.6 | 4 |
| Completeness [%] | 99.8 | 97 |
| Redundancy | 5.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M Lithium Sulfate 0.1 M TRIS:HCl pH 8.5 30% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
