4RTQ
Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoHcy and a 5-bp non-canonical site (GTTTA )
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.009, 62.918, 136.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.997 |
| R-factor | 0.1623 |
| Rwork | 0.161 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g1p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.760 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | GLRF |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.021 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.111 | 0.220 |
| Number of reflections | 26232 | |
| <I/σ(I)> | 15.9 | 9.8 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 289 | 28% PEG 4000, 80mM Magnesium acetate, 50mM sodium cacodylate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
