4RTO
Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with Sinefungin and with DNA Containing Proximal Pap Regulon Sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.936, 63.254, 143.211 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.855 - 2.690 |
R-factor | 0.2195 |
Rwork | 0.218 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g1p |
RMSD bond length | 0.002 |
RMSD bond angle | 0.483 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | GLRF |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.900 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.095 | 0.470 |
Number of reflections | 9617 | |
<I/σ(I)> | 11.4 | 3.7 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 5.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 289 | 16% PEG200, 100mM KCl, 10mM MgSO4, 100mM HEPES buffer, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |