4RTN

Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoHcy and with DNA Containing Proximal Pap Regulon Sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	36.201, 63.477, 144.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 2.590
R-factor	0.2069
Rwork	0.205
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g1p
RMSD bond length	0.003
RMSD bond angle	0.598
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	GLRF
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.044	2.680
High resolution limit [Å]	2.590	2.590
Rmerge	0.083	0.503
Number of reflections	10911
<I/σ(I)>	12	4.8
Completeness [%]	100.0	100
Redundancy	9	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	24% PEG200, 100mM KCl, 10mM MgSO4, 100mM HEPES buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K