4RTD
Escherichia coli alpha-2-macroglobulin activated by porcine elastase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97620 |
| Spacegroup name | H 3 |
| Unit cell lengths | 176.065, 176.065, 161.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.870 - 3.650 |
| R-factor | 0.18042 |
| Rwork | 0.177 |
| R-free | 0.23831 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 4.000 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.077 | 0.744 |
| Number of reflections | 20753 | |
| <I/σ(I)> | 13.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 26.6 | 26.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M potassium chloride, 25% SOKALAN CP7, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
