4RTC
Crystal structure of the green fluorescent variant, nowGFP, of the cyan Cerulean at pH 9.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.058, 51.210, 55.679 |
| Unit cell angles | 90.00, 100.01, 90.00 |
Refinement procedure
| Resolution | 27.420 - 1.350 |
| R-factor | 0.16238 |
| Rwork | 0.162 |
| R-free | 0.19262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.069 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.056 | 0.035 | 0.547 |
| Number of reflections | 67619 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 100.0 | 99.7 | 99.9 |
| Redundancy | 3.7 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 14mM KH2PO4, pH 4.8, 14% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
