4RTB
X-ray structure of the FeFe-hydrogenase maturase HydG from Carboxydothermus hydrogenoformans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-13 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.230, 92.230, 167.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.780 - 2.790 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.25500 |
Structure solution method | AND SAD |
Starting model (for MR) | 1r30 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.819 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.784 |
High resolution limit [Å] | 2.789 |
Number of reflections | 34198 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | Ammonium Sulfate 1M, HEPES 100 mM pH 7.5, 0.5% PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |