4RT5
The crystal structure of a glyoxalase/bleomycin resistance protein/dioxygenase protein from planctomyces limnophilus dsm 3776
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.130, 77.496, 45.827 |
| Unit cell angles | 90.00, 109.19, 90.00 |
Refinement procedure
| Resolution | 37.790 - 1.500 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 30528 | |
| <I/σ(I)> | 17.1 | 2.3 |
| Completeness [%] | 96.6 | 90.2 |
| Redundancy | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.2 M Magnesium Chloride, 0.1 M TRIS:HCl, pH 8.5, 30% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
