4RT4
Crystal structure of Dpy30 complexed with Bre2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.229, 75.229, 105.758 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.440 - 1.997 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g36 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 | 2.050 |
| High resolution limit [Å] | 1.997 | 2.580 | 2.000 |
| Rmerge | 0.069 | 0.120 | 0.396 |
| Number of reflections | 23016 | ||
| <I/σ(I)> | 35.1 | 18.3 | 2.4 |
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 11.3 | 11.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.1M Tris-HCl, pH 8.5, 2.0M NH4H2PO4, EVAPORATION, temperature 291K |
