4RSX
The structure of the effector protein from Pseudomonas syringae pv. tomato strain DC3000
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 65 |
Unit cell lengths | 79.769, 79.769, 96.472 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.738 - 2.303 |
R-factor | 0.162 |
Rwork | 0.158 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rsw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.016 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.161 | 0.082 | 0.652 |
Number of reflections | 15481 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 22.6 | 21.5 | 22.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M sodium citrate/citric acid, 10%(v/v) 2-propanol, 20%(w/v) polyethylene glycol 3350 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |