4RSX

The structure of the effector protein from Pseudomonas syringae pv. tomato strain DC3000

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 65
Unit cell lengths	79.769, 79.769, 96.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.738 - 2.303
R-factor	0.162
Rwork	0.158
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rsw
RMSD bond length	0.008
RMSD bond angle	1.016
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.161	0.082	0.652
Number of reflections	15481
<I/σ(I)>	5.1
Completeness [%]	99.9	99.5	100
Redundancy	22.6	21.5	22.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.1 M sodium citrate/citric acid, 10%(v/v) 2-propanol, 20%(w/v) polyethylene glycol 3350 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K