4RSW

The structure of the effector protein from Pseudomonas syringae pv. syringae strain 61

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97946, 0.97960, 0.97178
Spacegroup name	P 41
Unit cell lengths	62.522, 62.522, 156.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.740 - 1.900
R-factor	0.1964
Rwork	0.195
R-free	0.22640
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.096
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.126	0.080	0.962
Number of reflections	47054
<I/σ(I)>	6
Completeness [%]	99.6	96.7	100
Redundancy	14.8	12.4	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	56.43 %(v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K