4RS8
Apo structure of novel pNOB8 plasmid centromere binding protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979, 0.980, 0.965 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.300, 63.600, 86.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.150 - 2.290 |
R-factor | 0.2144 |
Rwork | 0.214 |
R-free | 0.25840 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.995 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.150 | |
High resolution limit [Å] | 2.290 | 2.290 |
Number of reflections | 15804 | |
Completeness [%] | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG1500, phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |