4RS6
Crystal structure of the C domain of Polo like Kinase II in Homo Sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | I 2 3 |
Unit cell lengths | 153.075, 153.075, 153.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.830 - 2.600 |
R-factor | 0.18891 |
Rwork | 0.186 |
R-free | 0.24724 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j7b |
RMSD bond length | 0.015 |
RMSD bond angle | 1.737 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.010 | 2.600 |
Rmerge | 0.160 | 0.150 | 0.860 |
Number of reflections | 17518 | ||
<I/σ(I)> | 27 | 1.5 | |
Completeness [%] | 88.0 | 100 | 100 |
Redundancy | 21.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 35%(v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |