4RS6

Crystal structure of the C domain of Polo like Kinase II in Homo Sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 2 3
Unit cell lengths	153.075, 153.075, 153.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.830 - 2.600
R-factor	0.18891
Rwork	0.186
R-free	0.24724
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j7b
RMSD bond length	0.015
RMSD bond angle	1.737
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.640
High resolution limit [Å]	2.600	7.010	2.600
Rmerge	0.160	0.150	0.860
Number of reflections	17518
<I/σ(I)>		27	1.5
Completeness [%]	88.0	100	100
Redundancy	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	35%(v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K