4RR3
Crystal structure of a recombinant EV71 virus particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.978030 |
Spacegroup name | P 42 3 2 |
Unit cell lengths | 350.599, 350.599, 350.599 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.438 - 3.103 |
R-factor | 0.2358 |
Rwork | 0.235 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 1.131 |
RMSD bond angle | 0.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.967 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 90.7 | 100 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1M Imidazole containing 1M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |