4RR2
Crystal structure of human primase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 86.195, 88.899, 94.682 |
Unit cell angles | 93.82, 96.57, 111.72 |
Refinement procedure
Resolution | 48.480 - 2.650 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 | 2.640 |
High resolution limit [Å] | 2.600 | 2.690 | 2.600 |
Rmerge | 0.040 | 0.485 | 0.653 |
Number of reflections | 72404 | ||
<I/σ(I)> | 27.8 | 2 | 1.53 |
Completeness [%] | 91.5 | 93.6 | 93.5 |
Redundancy | 3.8 | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 50 mM Tris-HCl pH 8.5, 7.5% v/v ethanol and 2 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 295K |