4RQ2
Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine (8-oxoG)and dCTP soaked with MnCl2 for 35 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2014-08-15 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.850, 82.290, 54.680 |
Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
Resolution | 46.640 - 2.200 |
R-factor | 0.19774 |
Rwork | 0.194 |
R-free | 0.26868 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rje |
RMSD bond length | 0.010 |
RMSD bond angle | 1.403 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.097 | 0.604 |
Number of reflections | 19135 | |
<I/σ(I)> | 7.7 | 2.3 |
Completeness [%] | 90.8 | 90.1 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM Acetate, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |