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4RQ2

Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine (8-oxoG)and dCTP soaked with MnCl2 for 35 s

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 31-ID
Synchrotron siteAPS
Beamline31-ID
Temperature [K]193
Detector technologyCCD
Collection date2014-08-15
DetectorRAYONIX MX225HE
Wavelength(s)0.97931
Spacegroup nameP 1 21 1
Unit cell lengths49.850, 82.290, 54.680
Unit cell angles90.00, 110.68, 90.00
Refinement procedure
Resolution46.640 - 2.200
R-factor0.19774
Rwork0.194
R-free0.26868
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3rje
RMSD bond length0.010
RMSD bond angle1.403
Data reduction softwareMOSFLM
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0073)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]46.6402.270
High resolution limit [Å]2.2002.200
Rmerge0.0970.604
Number of reflections19135
<I/σ(I)>7.72.3
Completeness [%]90.890.1
Redundancy3.63.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP829550 MM IMIDAZOLE, 350 MM SODIUM Acetate, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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