4ROP

Crystal structure of enolase from Synechococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127092
Spacegroup name	I 4 2 2
Unit cell lengths	164.282, 164.282, 75.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.780 - 2.050
R-factor	0.20466
Rwork	0.203
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e9i
RMSD bond length	0.010
RMSD bond angle	1.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.115	0.631
Rpim	0.038	0.207
Number of reflections	30631	2261
<I/σ(I)>	9	2.2
Completeness [%]	98.7
Redundancy	10.1
CC(1/2)	0.998	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	10-15% PEG200, 150-200 mM calcium acetate, 0.1 M HEPES, pH 7.5