4RO3
1.8 Angstrom Crystal Structure of the N-terminal Domain of Protein with Unknown Function from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.065, 48.576, 49.399 |
| Unit cell angles | 90.00, 96.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.16238 |
| Rwork | 0.161 |
| R-free | 0.19573 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.406 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.577 |
| Number of reflections | 21028 | |
| <I/σ(I)> | 40 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | Protein: 7.0 mg/ml, 0.3M Sodium chloride, 0.01M HEPES (pH 7.5), + Thermolysin; Screen: 0.2M Lithium sulfate, Bis-Tris 0.1M (pH 5.5), 25% (w/v) PEG 3350; Cryo: 5% Glycerol in screen solution, then Paraton, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
