4RO0
Crystal structure of MthK gating ring in a ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 166.339, 231.680, 197.979 |
Unit cell angles | 90.00, 94.58, 90.00 |
Refinement procedure
Resolution | 49.260 - 3.180 |
R-factor | 0.26385 |
Rwork | 0.263 |
R-free | 0.30065 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.103 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.260 | 3.310 |
High resolution limit [Å] | 3.180 | 3.200 |
Rmerge | 0.161 | 0.742 |
Number of reflections | 229804 | |
<I/σ(I)> | 10.8 | 1.8 |
Completeness [%] | 92.8 | 93.4 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |