4RNH
PaMorA tandem diguanylate cyclase - phosphodiesterase, c-di-GMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.300, 81.300, 128.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.700 - 2.450 |
| R-factor | 0.1873 |
| Rwork | 0.184 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.566 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.700 | 2.520 |
| High resolution limit [Å] | 2.450 | 2.460 |
| Rmerge | 0.107 | 0.746 |
| Number of reflections | 18513 | |
| <I/σ(I)> | 14.5 | 2.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M sodium thiocyanate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
