4RMU
Crystal structure of the D76E Beta-2 Microglobulin mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.401, 28.866, 57.870 |
Unit cell angles | 90.00, 124.17, 90.00 |
Refinement procedure
Resolution | 47.881 - 1.400 |
R-factor | 0.1661 |
Rwork | 0.163 |
R-free | 0.19210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yxf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.637 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.881 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.050 | 0.104 |
Number of reflections | 20395 | |
<I/σ(I)> | 19.2 | 9.4 |
Completeness [%] | 94.3 | 82 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 24% PEG4000, 15% glycerol, 0.2 M ammonium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |