4RMP

Crystal structure of allophycocyanin from marine cyanobacterium Phormidium sp. A09DM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-06-17
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	101.242, 101.242, 193.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.226 - 2.506
R-factor	0.1617
Rwork	0.158
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kn1
RMSD bond length	0.008
RMSD bond angle	1.648
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.640
High resolution limit [Å]	2.506	2.506
Rmerge	0.109	0.557
Number of reflections	13362
<I/σ(I)>	22.8	5.3
Completeness [%]	99.9	99.8
Redundancy	10.8	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	288	20% (w/v) PEG 6000, 0.1M bicine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K