4RMM
Crystal Structure of the Q7NVP2_CHRVO protein from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR191
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97912 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 47.065, 47.065, 120.816 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.855 - 2.200 |
R-factor | 0.245 |
Rwork | 0.244 |
R-free | 0.28000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.649 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX (D/E) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.061 | 0.618 |
Number of reflections | 13206 | |
<I/σ(I)> | 36.8 | 2.07 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under parafin oil | 7 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Crystallization solution: 20% PEG 4000, 0.1M potassium nitrate, 0.1M Bis-Tris propane, pH 7.0, microbatch under parafin oil, temperature 291K |