4RML

Crystal structure of the Olfactomedin domain of latrophilin 3 in C2221 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	C 2 2 21
Unit cell lengths	78.806, 96.819, 79.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 1.601
R-factor	0.1766
Rwork	0.175
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.068
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	1.640
High resolution limit [Å]	1.550	1.550
Number of reflections	78279
<I/σ(I)>	12.59	1.68
Completeness [%]	98.8	61.7
Redundancy		2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbach under-oil	9	293	15-25 % PEG 8000, 50-300 mM MgCl2, 100 mM TAPS pH 9.0 , Microbach under-oil, temperature 293K