4RLC
Crystal structure of the N-terminal beta-barrel domain of Pseudomonas aeruginosa OprF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 52.009, 26.779, 54.892 |
| Unit cell angles | 90.00, 95.79, 90.00 |
Refinement procedure
| Resolution | 54.610 - 1.600 |
| R-factor | 0.19065 |
| Rwork | 0.189 |
| R-free | 0.22879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.040 |
| Data reduction software | XDS |
| Data scaling software | XDS (/ XSCALE) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.612 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 20209 | |
| Completeness [%] | 99.6 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 292 | 33% PEG200, 0.2 M ammonium sulfate, 0.02 M sodium chloride, 0.02 M sodium citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
