4RL3
Crystal Structure of the Catalytic Domain of a family GH18 Chitinase from fern, Peteris ryukyuensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 41 |
| Unit cell lengths | 78.626, 78.626, 167.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.570 |
| R-factor | 0.15093 |
| Rwork | 0.150 |
| R-free | 0.16488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sim |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.162 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.170 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.068 | 0.096 |
| Number of reflections | 70282 | |
| <I/σ(I)> | 68.92 | 32.86 |
| Completeness [%] | 99.7 | 95.6 |
| Redundancy | 7.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 298 | 0.2M Magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3350, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
