4RKN
Wolinella succinogenes octaheme sulfite reductase MccA, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | PIXEL |
| Collection date | 2012-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7389 |
| Spacegroup name | H 3 |
| Unit cell lengths | 186.472, 186.472, 232.836 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.260 - 2.100 |
| R-factor | 0.13666 |
| Rwork | 0.135 |
| R-free | 0.17517 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.921 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.260 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.102 |
| Number of reflections | 175765 |
| <I/σ(I)> | 17.3 |
| Completeness [%] | 99.8 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 15% PEG 3350, 0.1 M CHES/NaOH, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
