4RKL
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.153, 60.402, 38.472 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.660 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.975 |
| Data reduction software | DENZO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.015 | 0.117 |
| Number of reflections | 16877 | |
| <I/σ(I)> | 6.56 | 6.56 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.39 | 5.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K |
