4RKI
Crystal structure of sliding beta clamp from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-06-27 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.769 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.534, 65.886, 82.723 |
Unit cell angles | 90.00, 115.79, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.050 |
R-factor | 0.21658 |
Rwork | 0.215 |
R-free | 0.24729 |
Structure solution method | MR SAD |
Starting model (for MR) | 2awa |
RMSD bond length | 0.006 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 52832 | |
<I/σ(I)> | 29.43 | 2.05 |
Completeness [%] | 99.5 | 95 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | 6% Peg20000, 20% Peg550MME, 0.1M Hepes+MOPS pH7.3, 0.2M Ammonium citrate dibasic, 10mM MgCl2+10mM SrCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |