4RKH
Structure of the MSL2 CXC domain bound with a specific MRE sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97913 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.371, 50.998, 124.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.752 - 2.000 |
R-factor | 0.2002 |
Rwork | 0.197 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.118 | 0.620 |
Number of reflections | 21109 | |
<I/σ(I)> | 22.1 | |
Completeness [%] | 94.9 | 83.7 |
Redundancy | 6.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES-Na (pH 7.5), 10% PEG 3350 (w/v), 0.2M proline, VAPOR DIFFUSION, HANGING DROP, temperature 293K |