4RKF
Drosophila melanogaster Rab3 bound to GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.230, 80.470, 123.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.269 - 1.500 |
| R-factor | 0.1585 |
| Rwork | 0.157 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rab |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 60482 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 28% (v/v) PEG 200, 5% (w/v) PEG 3000; 100 mM MES buffer at pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
