4RKC
Psychrophilic aromatic amino acids aminotransferase from Psychrobacter sp. B6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8020 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.687, 62.106, 85.895 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.190 |
| R-factor | 0.14517 |
| Rwork | 0.142 |
| R-free | 0.20316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FSL and 3QN6 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.048 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.091 | 0.386 |
| Number of reflections | 39647 | |
| <I/σ(I)> | 16.67 | 3.72 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M Mg(NO3)2, 20% PEG3350, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
