4RK5
Crystal structure of LacI family transcriptional regulator from Lactobacillus casei, Target EFI-512911, with bound sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-25 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | H 3 2 |
Unit cell lengths | 108.754, 108.754, 125.763 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.100 - 1.350 |
R-factor | 0.11003 |
Rwork | 0.109 |
R-free | 0.14093 |
Structure solution method | MR |
RMSD bond length | 0.020 |
RMSD bond angle | 1.929 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.051 | 0.880 |
Number of reflections | 62512 | |
<I/σ(I)> | 39 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.1 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | protein in 10 mM Bis-Tris, 500 mM sodium chloride, 10% glycerol, 5 mM DTT, TEV protease (1:100 ratio), reservoir: 0.16 M calcium acetate, 0.08 M sodium cacodylate, pH 6.5, 14.4% PEG8000, 20% glycerol, cryoprotectant = reservoir + 100 mM sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 294K |