4RK0
Crystal structure of LacI family transcriptional regulator from Enterococcus faecalis V583, Target EFI-512923, with bound ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 |
Unit cell lengths | 36.919, 77.234, 91.805 |
Unit cell angles | 87.72, 82.57, 84.33 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.17168 |
Rwork | 0.170 |
R-free | 0.22271 |
Structure solution method | MR |
Starting model (for MR) | 3ctp |
RMSD bond length | 0.012 |
RMSD bond angle | 1.520 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.097 | 0.690 |
Number of reflections | 93664 | |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 97.7 | 95.7 |
Redundancy | 4.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | protein in 10 mM Bis-Tris, 500 mM sodium chloride, 10% glycerol, 5 mM DTT, 10 mM D-glucose, reservoir: 0.17 M sodium acetate, 0.085 M Tris-HCl, 8.5 25.5% w/v PEG4000, 15% w/v glycerol, cryoprotectant = reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 294K |